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22h
上海富衡 | 293T 细胞 G418 筛选实用指南
筛选浓度确定 :293T 细胞对 G418 较为敏感,筛选浓度多在 200 - 800μg/mL 区间,但最适配浓度需预实验摸索。准备多组六孔板,每孔铺适量且等量 293T 细胞(约 5×10⁵个 /mL),待细胞贴壁、状态良好(24 ...
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