These basic processes, known collectively as the cell cycle ... This process involves replication of the cell's chromosomes, segregation of the copied DNA, and splitting of the parent cell's ...

New research sheds light on how cells repair damaged DNA. For the first time, the team has mapped the activity of repair proteins in individual human cells. The study demonstrates how these proteins ...

New research from the Kind Group at the Hubrecht Institute sheds light on how cells repair damaged DNA. For the first time, ...

When the fork stalls due to an obstacle on the DNA (Figure 1), the cell activates the replication checkpoint to send a signal to arrest the progression of the cell cycle. Another example of ...

Collected fractions are analysed by PCR for genetic analysis after immunoprecipitation of DNA via incorporated ... A dynamic switch in the replication timing of key regulator genes in embryonic stem ...

Nucleotides are abundant in the cell's nucleus. Nucleotides are the units which, when linked sugar to phosphate, make up one side of a DNA ladder. During DNA replication, special enzymes move up ...

Replication of DNA DNA replication is the process by which a cell makes an identical copy of its DNA. Gene expression Only a fraction of the genes possessed by any particular cell will be expressed.

Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

The precision of this process depends in large measure upon stringent regulatory mechanisms that couple DNA replication to cell cycle progression. Initiation must be triggered at the appropriate time ...

The primary G1/S cell cycle checkpoint regulates eukaryotic cells’ commitment to enter DNA synthesis (S phase) and serves as a stop/go decision prior to cell division. Cyclin-dependent kinase ...

In healthy DNA replication, at first ... one enzyme named CDK1 prevents the loading of CENP-A during most of the cell cycle, ...