The mechanism of division in bacteria differs from that of eukaryotes, and the control of their cell cycle is also somewhat different, although again it is linked with DNA replication. Although ...

(2001) demonstrated that the budding yeast protein Mrc1 is the replication checkpoint transducer ... However, prolonged RF stalling leads to DNA damage; alternatively, the fork may collapse ...

Nucleotides are abundant in the cell's nucleus. Nucleotides are the units which, when linked sugar to phosphate, make up one side of a DNA ladder. During DNA replication, special enzymes move up ...

This study examines the impact of DNA methylation on CTCF binding in two cancer cell lines. Increased CTCF binding sites are enriched in gene bodies, and associate with nuclear speckles, indicating a ...

The determination of the percentage of cells in S-phase is dependent upon the detection of a thymidine analogue, bromodeoxyuridine (BrdU), which when added to culture medium is incorporated into DNA ...

Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

By inferring proliferation rates from single-cell genomics, SPRINTER enhances understanding of tumor evolution and identifies ...

The primary G1/S cell cycle checkpoint regulates eukaryotic cells’ commitment to enter DNA synthesis (S phase) and serves as a stop/go decision prior to cell division. Cyclin-dependent kinase ...

Replication of DNA DNA replication is the process by which a cell makes an identical copy of its DNA. Gene expression Only a fraction of the genes possessed by any particular cell will be expressed.

It tells the cell how, where and when to 'read' and use the information in the DNA. It is important that both the DNA and its ...