The mechanism of division in bacteria differs from that of eukaryotes, and the control of their cell cycle is also somewhat different, although again it is linked with DNA replication. Although ...

Recent work has clarified how higher eukaryotic cells 'licence' DNA replication precisely once per cycle. An inhibitor, geminin, prevents replication before mitosis by inhibiting the replication ...

Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

Nucleotides are abundant in the cell's nucleus. Nucleotides are the units which, when linked sugar to phosphate, make up one side of a DNA ladder. During DNA replication, special enzymes move up ...

The determination of the percentage of cells in S-phase is dependent upon the detection of a thymidine analogue, bromodeoxyuridine (BrdU), which when added to culture medium is incorporated into DNA ...

The primary G1/S cell cycle checkpoint regulates eukaryotic cells’ commitment to enter DNA synthesis (S phase) and serves as a stop/go decision prior to cell division. Cyclin-dependent kinase ...

Replication of DNA DNA replication is the process by which a cell makes an identical copy of its DNA. Gene expression Only a fraction of the genes possessed by any particular cell will be expressed.

When cells express specific genes that characterise a certain type of cell we say that a cell has become differentiated. Structure and replication of DNA DNA is the molecule that holds the ...

"If there is a problem with DNA packaging, the cell quickly senses this issue. But instead of triggering a typical stress response, the cell responds by slowing down its cycle of growth and ...