A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes.
It was found that RNA removal improves the throughput of subsequent ion-exchange columns for plasmid DNA, reducing the resolution required to produce RNA-free plasmid. Figure 4: FPLC anion ...
Zhong, L. Y. , Srirangan, K. , Scharer, J. , Moo-Young, M. , Fenner, D. , Crossley, L. , Honeyman, C. H. , et al. (2011). Developing an RNase-free bioprocess to ...
Objectives To compare the transfection efficiency of three ADRB1 RNA interference plasmid pAAV-ZsGreen-ADRB1-shRNA transfected to H9c2 myocardial cell mediated by Lipofectamine 2000. Results ...
Topics to be covered Engineering a conjugative plasmid to carry a CRISPR-based system and using this tool to remove antimicrobial resistance genes from microbial communities Developing a cell-free ...