AZoNano on MSN1mon
Destabilized Near-Infrared Nanobodies for Enhanced GFP Imaging
In a recent Nature Communications article, researchers introduced destabilized near-infrared (NIR) fluorescent nanobodies ...
Nature5y
Colouring in cells
This year's Nobel Prizes mark the most significant technological advance in cell biology, GFP et al ... interactions and allows the design of biosensors that can, for example, monitor the ...
eLife1mon
CUTS RNA Biosensor for the Real-Time Detection of TDP-43 Loss-of-Function
(A) Mean intensity quantification of GFP signal intensity from live imaging, presented as fold change from mock. (B) Relative pixel quantification of GFP normalized to total protein (Ponceau S), ...
Nature2mon
Training GFP to fold
A method to select for proteins that are highly efficient at folding yields 'superfolder' GFP, an optimized GFP for use in protein fusions. Fusion proteins are powerful tools for the elucidation ...
eLife1mon
CUTS RNA Biosensor for the Real-Time Detection of TDP-43 Loss-of-Function
Lastly, we replaced the GFP cassette with the wildtype TARDBP coding sequence (CUTS-TDP43) to highlight the utility of CUTS biosensor as a regulator of a payload in response to TDP-43 loss of function ...