Thus, single-primer PCR amplifies the genomic library and produces double-stranded DNA fragments with complementary adaptors. In addition to the complementary tags, two independent tags can be ...

Set up two reactions for digestion of genomic DNA: Incubate the two reactions at 37 °C for 3 h. Extract the reactions with phenol/chloroform (1:1, v/v), recover the DNA by precipitation with ...

KG) is popular among scientists for NGS library preparation because it meets all necessary standards. Four genes from human genomic DNA (Promega Cooperation) were targeted via PCR (Table 1 ...

This poster outlines the optimal parameters for PCR-free WGS library preparation, evaluating eight commercially available ...

We will isolate genomic DNA and produce restriction fragments ... Such fragments would be useful for producing a partial genomic library (subgenomic library), from which the gene could be cloned.

科技日报北京9月1日电 （记者张梦然）DNA因其标志性的双螺旋结构而广为人知。但澳大利亚加文医学研究所科学家发现，人类基因组还含有超过5万个不寻常的结状DNA结构，称为i-基序。最新一期《EMBO》杂志发表了这些独特DNA结构的第一张综合图谱，揭示了它们在 ...

Genome and mutations DNA replication is carefully controlled to preserve the genetic information. However, changes in the genome do sometimes occur. These changes are known as mutations.

Determining the number of copies of a double stranded DNA template (be it genomic DNA, plasmid or an amplified fragment) is essential for many genetic quantification applications in research and ...

This valuable study addresses potential roles of the master regulator of X chromosome inactivation, the Xist long non-coding RNA, in autosomal gene regulation. Using data from mouse cells, the authors ...

The human genome is too long to be sequenced as one continuous string by current technology – so short-read sequencing breaks DNA into short fragments that are amplified and then sequenced to ...

The R-loop is a common transcriptional by-product that consists of an RNA-DNA duplex joined to a displaced strand of genomic DNA. While the effects of R-loops on health and disease are well ...

scientists can dispatch a synthetic replacement part to any location in a genome made of billions of nucleotides. When it reaches its destination, the Cas9 enzyme snips out the unwanted DNA sequence.