ATP-dependent DNA ligases are found in all eukaryotic species. Mammalian cells contain four DNA ligase isozymes. Amino acid-sequence comparisons suggest that a core catalytic domain common to all ...

Also unknown are whether the ubiquitin-ligase activity requires only CHIP ... avenues for the degradation of misfolded proteins in the cell, as degradation of Hsp70-bound ApoB is not affected ...

We’ve shown that: (i) each component catalytic module of tRNA ligase is essential for yeast cell growth; (ii) mutations in the active sites of the CPD, kinase, and ligase domains are lethal in vivo; ...

E3 ligases transfer ubiquitin to target proteins for degradation, requiring precise recognition of both ubiquitin and their substrate. This specificity makes E3 ligases valuable drug targets for ...