All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt ... If using a column-based PCR cleanup kit, make sure that the procedure is designed to capture products ...