Baculovirus-mediated gene transfer to human and mouse pancreatic islet dissociated cells. Using the recombinant baculovirus BacCMV-GFP, enhanced GFP (EGFP) expression was observed in both primary ...
In some experiments, 500 nmol/l staurosporine or 0.1 and 1 mmol/l streptozotocin were added to mouse islets for 48 h to induce cell death. Cell death was confirmed by TUNEL (Roche). For acute ...
There is no reciprocal identity change of mouse islet cells after HFD treatment while β-cells undergo complex identity changes through the recovery after STZ treatment.
Shaun Coughlin and colleagues recently reported that mouse islets express high levels of at least 28 different GPCRs (Regard et al. 2007). Table 1 lists some of the GPCRs expressed by beta cells ...