There is no reciprocal identity change of mouse islet cells after HFD treatment while β-cells undergo complex identity changes through the recovery after STZ treatment.
For assessment of the organization of α- and β-cells within the islets, 10 sections per animal were labeled with mouse monoclonal antibodies to glucagon (Sigma) or insulin, respectively ...
In some experiments, 500 nmol/l staurosporine or 0.1 and 1 mmol/l streptozotocin were added to mouse islets for 48 h to induce cell death. Cell death was confirmed by TUNEL (Roche). For acute ...