PCR—the workhorse of modern molecular biology—is charging forward using both conventional and digital methods to explore single cells and even single molecules. Nathan Blow reports. In late ...

此外，蛋白在纯化过程中其构象可能发生改变，所获得的药物-靶标相互作用信息不能反映活细胞内的真实情况。而且很多蛋白很难重组表达纯化，进而限制了利用生物物理分子互作技术鉴定化合物和蛋白的相互作用。这时，也可以采用 细胞热迁移技术 (cell ...

PCR, or Polymerase Chain Reaction, is a revolutionary molecular biology technique that allows for the rapid amplification of specific DNA sequences. It is an essential tool in various fields, ...

Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR. Figure 2: Ethidium bromide–stained agarose gels reveal the absence of size competition and chimeric produc ...

1. Prepare a 40 ml 1 % agarose gel in 0.5X TBE, containing 0.5 µg/ml ethidium bromide. Each gel will be shared by 2 groups on the same bench. 3. Each group will load PCR and control samples on one ...

基于论文中的研究结果，研究提出了一个牡丹与芍药杂交形成伊藤杂种牡丹的遗传模型（图4）：在牡丹的减数分裂过程中，由于减数分裂Ⅰ期同源染色体未能正常分离，产生了含有两套异质性染色体的2n雄配子。该配子与芍药通过正常减数分裂产生的1n雌配子结合，形成了三倍 ...

As a Bucknell cell biology/biochemistry major, you'll use leading-edge equipment and techniques — like molecular cloning, PCR amplification and cell culture — that you otherwise might not touch until ...

When the first thermal cyclers for the polymerase chain reaction came out in the 1980s, they were as expensive as a market driven by grant money could make them. Things haven’t got much better ...

▎药明康德内容团队编辑  导师简介：袁野，中国科学院过程工程研究所生物药制备与递送重点实验室副主任。获得上海市浦江人才计划、东方英才、青年五四奖章。主持多项省部级以上科研项目，包括国家自然科学基金、科技部重点研发计划项目课题等。以第一/通讯作者身份在多个Nature子刊、PNAS、Genome Biology、PLOS ...

Oct. 11, 2024 — Researchers have developed a new method to recreate a robust and functional human immune system in mice using mononuclear cells from the cord blood. This new kind of mice, that ...

Researchers at BITS Pilani have developed a groundbreaking device capable of rapidly detecting harmful bacteria, including ...