In Sanger sequencing, the DNA to be sequenced serves as a template for DNA synthesis. A DNA primer is designed to be a starting point for DNA synthesis on the strand of DNA to be sequenced.

At the Nevada Genomics Center we offer DNA sequencing using dye-terminator Sanger sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A Sanger sequencing reaction is run with ...

0403410101. Fien, K. et al. 2004. Primer utilization by DNA polymerase alpha-primase is influenced by its interaction with Mcm10p. The Journal of Biological Chemistry 279 16144–16153.