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Use of paired Cas9-NG nickase and truncated sgRNAs for single-nucleotide microbial genome ...
Mutagenic PCR products of 1 kb size (500 ng) and 5′-end 2-nucleotide (nt)-truncated dual sgRNA plasmid (200 ng) were electroporated simultaneously into the SH169 cells carrying the pKD46 plasmid ...
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Frontiers in Bioengineering and Biotechnology
Impact and progress Frontiers' impact Progress Report 2022 All progress reports ...