For many years this was the world that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) inhabited. Seemingly relegated to serving as a reliable loading control for western blots and other such ...

生物通首页 > 今日动态> 专题总汇>《蛋白质印迹手册》完整版 Western blotting技术作为生物实验室中最给力的工具之一，一直被研究人员广泛使用。默克密理博公司的《蛋白质印迹手册》（Protein Blotting Handbook）详细介绍了western blotting的原理、操作和技巧，无论是对 ...

加入兔二抗（Cat＃KC-RB-035）时同时加入HRP标记的抗GAPDH单抗（稀释比例为1：10,000，Cat＃KC-5G5）。采用化学发光试剂盒（康成生物Cat＃KC-420）及X胶片曝光显影。一次反应即可同时检测目的蛋白（Akt）与内参GAPDH的含量。 右图：A-E分别表示细胞株ELC、U937、HSC、SPCA ...

Western blotting forms the bedrock of many protein studies, with the technique cited in 9% of all protein related papers. The technique had its 40th birthday in 2019, an event that was widely ...

Department of Physiology, Harold Hamm Diabetes Center, The University of Oklahoma Health Science Center, Oklahoma City, United States ...

POLK expression in neurons and report an important observation that POLK exhibits an age-dependent change in subcellular localization, from the nucleus in young tissue to the cytoplasm in old tissue.

Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...

Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...

In a study led by researchers at the NUS Yong Loo Lin School of Medicine, extracellular vesicles loaded with customizable ...

The global western blotting market is on a steady growth trajectory, with market valuation expected to rise from USD 980.1 million in 2023 to USD 1,753.4 million by 2033, marking a robust CAGR of 6% ...

The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...