Avoid fusions to the GFP fragments at the site of protein-protein interaction and consider optimizing the spacer length between the fusions and GFP fragments, if necessary. In the absence of ...
This work will be of high interest to a broad swathe of scientists studying native macromolecules present at low concentrations in cells. Cryo-EM single-particle analyses typically require target ...
Researchers recently developed a new approach for identifying new cellular RNA targets of snoRNAs. They uncovered thousands of previously unknown targets for snoRNAs in human cells and mouse brain ...
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