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4 天
上海富衡 | 293T 细胞 G418 筛选实用指南
筛选浓度确定 :293T 细胞对 G418 较为敏感,筛选浓度多在 200 - 800μg/mL 区间,但最适配浓度需预实验摸索。准备多组六孔板,每孔铺适量且等量 293T 细胞(约 5×10⁵个 /mL),待细胞贴壁、状态良好(24 ...
众播育儿 on MSN
4 天
293T 细胞 G418 筛选,操作要点详述
在细胞生物学研究领域,利用 G418 对 293T 细胞进行筛选是常见操作,旨在精准获取稳定转染特定基因的细胞株,以下详述浓度与操作要点。 筛选浓度确定:293T 细胞对 G418 较为敏感,筛选浓度多在 200 - 800μg/mL ...
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