Mutagenic PCR products of 1 kb size (500 ng) and 5′-end 2-nucleotide (nt)-truncated dual sgRNA plasmid (200 ng) were electroporated simultaneously into the SH169 cells carrying the pKD46 plasmid ...
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The Cas12a protein was combined with crRNA to form a CRISPR-Cas12a/crRNA complex (Figure 1B) in advance to stabilise the CRISPR detection process. CRISPR-Cas12a/crRNA complexes contained 100 nM crRNA ...