Methods: In this study, we established a reverse genetics system for a local SVA isolate and used it to rescue a recombinant SVA, rSVA-eGFP, expressing the enhanced green fluorescent protein (eGFP) by ...
which contains the N-terminal 70 amino acids of the phage P1 RepA protein followed by green fluorescent protein (GFP) (Hoskins et al., 2000). The N-terminal sequence of RepA serves as a binding site ...
At a minimum, a hand-held blue or ultraviolet light source and a high-quality color digital camera can be used to illuminate the blot and capture images of the fluorescent protein bands.
Protein-rich foods have become increasingly popular among people today. Certain green vegetables offer healthy protein alternatives to beef, chicken, and seafood. It is a nutrient-dense choice ...