Self-cleaving ribozymes are RNA molecules that catalyze the cleavage of their own phosphodiester backbones. These ribozymes are found in all domains of life and are also a tool for biotechnical and ...
The parental strain DBY12394 (Mcisaac et al., 2013) (GAL2 + s288c repaired HAP1, ura3∆, leu2∆0::ACT1pr-Z3EV-NatMX) was transformed with a polymerase chain reaction (PCR) product (KanMX-Z3EVpr) to ...