详解CRISPR/Cas系统与其应用 - 知乎 - 知乎专栏
CRISPR/Cas系统作为一个革新性的强大基因组编辑工具的出现改变了这一境况, 可以说是颠覆了基因组编辑这个领域. CRISPR的全名是 (clustered regularly interspaced short palindromic repeats), 中文意思是成簇的, 规律间隔的,短回文重复序列. Cas 所指的是 (CRISPR-associated). 与上述的 ...
CRISPR - Wikipedia
The CRISPR array is made up of an AT-rich leader sequence followed by short repeats that are separated by unique spacers. [78] CRISPR repeats typically range in size from 28 to 37 base pairs (bps), though there can be as few as 23 bp and as many as 55 bp. [ 79 ] Some show dyad symmetry , implying the formation of a secondary structure such as a ...
最全CRISPR系统介绍（张锋等大牛执笔，14张图看懂CRISPR全部 …
第1类CRISPR-Cas系统利用多蛋白效应复合物，而第2类CRISPR-Cas系统利用单蛋白效应物。 基于不同的效应蛋白家族，第2类系统可分为三大类和九个亚型。 第十亚型（V-U）包括许多假定的系统，其免疫（或可能是调节）功能仍有待证明。
基因组CRISPR序列及Cas酶预测 - 知乎 - 知乎专栏
CRISPR也即Clustered regularly interspaced shortpalindromicrepeats（成簇的、规律间隔的、短回文、重复序列），由回文重复序列repeats及其间隔序列spacer组成，是大多数细菌及古细菌中的一种获得性免疫方式。. 在CRISPR特征序列附近还有一些CRISPR-associated基因，编码一系 …
Molecular mechanisms of CRISPR–Cas spacer acquisition
2018年8月31日 · The CRISPR array comprises a series of direct, semi-palindromic repeats intercalated with variable spacer sequences of foreign origin. The array is preceded by an AT-rich leader sequence.
Multiplexed CRISPR technologies for gene editing and ... - Nature
2020年3月9日 · In this review, we highlight strategies to create and process synthetic gRNA arrays in a multitude of organisms, emphasize the utility of inducible and orthogonal CRISPR–Cas technologies, and...
Novel CRISPR–Cas Systems: An Updated Review of the Current …
2021年3月24日 · The CRISPR array consists of short, direct repeats bordered with spacers. The direct repeats are nucleotide sequences in the genome with identical sequence and length. The sequences of direct repeats can be similar in related species, but overall diversity among the species is wide.
Multiplexed genome engineering by Cas12a and CRISPR arrays
2019年8月12日 · A single transcript encoding Cas12a and an array of CRISPR RNAs enables multiplexed genome engineering, from multiple knockouts to transcriptional activation or repression to orthogonal ...
CRISPR-Cas: biology, mechanisms and relevance - PMC
2016年11月5日 · The CRISPR-Cas systems are composed of a cas operon (blue arrows) and a CRISPR array that comprises identical repeat sequences (black rectangles) that are interspersed by phage-derived spacers (coloured rectangles). Upon phage infection, a sequence of the invading DNA (protospacer) is incorporated into the CRISPR array by the Cas1–Cas2 complex.
The Biology of CRISPR-Cas: Backward and Forward - Cell Press
2018年3月9日 · The CRISPR array serves as a genetic memory bank, and spacer acquisition into the array is accomplished in several steps: the detection of an MGE, protospacer selection, protospacer processing, and spacer integration into the CRISPR array.