The impact of DNA intercalators on DNA and DNA-processing enzymes ...
2015年6月18日 — DNA intercalators are widely used as fluorescent probes to visualize DNA and DNA transactions in vivo and in vitro. It is well known that they perturb DNA structure and stability, which can...
Rotation tracking of genome-processing enzymes using DNA ... - Nature
2019年7月17日 — Methods that directly measure DNA rotation, such as rotor bead tracking 1,2,3, angular optical trapping 4 and magnetic tweezers 5, have helped to unravel the action mechanisms of a range of...
Enzymes that keep DNA under control - PMC - National Center …
2001年4月4日 — Life demands not only the faithful and controlled replication of DNA but, in addition, many other enzymatic processes involving DNA, including topoisomerase action, recombination, repair, restriction and modification.
Engineering DNA processing enzymes for the postgenomic era
2009年8月1日 — In this review, I highlight some of the recent advances made to generate DNA processing enzymes to address the challenges put forward in the postgenomic era. Because of the pivotal role in DNA synthesis, DNA polymerases play a central role among the DNA processing enzymes.
DNA Replication Mechanisms - Molecular Biology of the Cell
To produce a continuous DNA chain from the many DNA fragments made on the lagging strand, a special DNA repair system acts quickly to erase the old RNA primer and replace it with DNA. An enzyme called DNA ligase then joins the 3′ end of the new DNA fragment to the 5′ end of the previous one to complete the process ( Figures 5-13 and 5-14 ).
Nucleosome dynamics regulates DNA processing - Nature
2013年6月2日 — We find that efficient resection by the Sgs1–Dna2–dependent machinery requires a nucleosome-free gap adjacent to the DSB. Resection by Exo1 is blocked by nucleosomes, and processing activity can...
A simple and general approach to generate photoactivatable DNA ...
2022年4月4日 — Here, we introduce a simple and general method to design light-start DNA processing enzymes. In order to prove its versatility, we applied our method to three DNA polymerases commonly used in biotechnology, including the Phi29 (mesophilic), Taq, and Pfu polymerases, and one restriction enzyme.
Biologically Inspired Synthetic Enzymes Made from DNA - Cell Press
The overall process of in vitro selection constitutes a cycle that typically must be repeated for multiple rounds, until the active molecules are sufficiently represented to enable their identification by molecular cloning and sequencing. ... Brothers in arms: DNA enzymes, short interfering RNA, and the emerging wave of small-molecule nucleic ...
Functional hierarchy of PCNA-interacting motifs in DNA processing enzymes
Numerous eukaryotic DNA processing enzymes, such as DNA polymerases and ligases, bind the processivity factor PCNA, which acts as a platform to recruit and regulate the binding of enzymes to their DNA substrate. Multiple PCNA-interacting …
Structural basis for DNA 3′-end processing by human tyrosyl-DNA ...
2018年1月2日 — Tyrosyl-DNA phosphodiesterase (Tdp1) is a DNA 3′-end processing enzyme that repairs topoisomerase 1B-induced DNA damage. We use a new tool combining site-specific...