CRISPR-Cas13系统的分类、功能及应用 - 知乎 - 知乎专栏
Cas13蛋白都是由多结构域组成的单一蛋白，具有识别crRNA、切割RNA、甚至是切割pre-crRNA的功能。. Cas13蛋白具有2个标志性的HEPN结构域，2个R-XXXX-H保守基序是HEPN结构域的核酸酶活性位点。. 据我所知，大部分Cas13家族都是基于R-XXXX-H基序特征及crRNA相关特 …
CRISPR-Cas13: A new technology for the rapid detection of …
2022年10月28日 · In 2016, East-Seletsky et al. (2016) developed a novel CRISPR-Cas13a-based method for detecting target RNAs by introducing RNA fluorescent reporter molecules that are connected to both fluorescent and quenching groups. The activated Cas13a not only specifically cleaves the target RNA, but it also cleaves the RNA fluorescent reporter molecules ...
Model-directed generation of artificial CRISPR–Cas13a guide RNA ...
2024年10月11日 · Model-directed generative design is applied to CRISPR–Cas13a guide RNAs, outperforming natural sequences. CRISPR guide RNA sequences deriving exactly from natural sequences may not perform ...
The CRISPR-Cas13a Gemini System for noncontiguous target …
2024年4月4日 · Here we propose a noncontiguous target RNA activation paradigm of Cas13a and establish a CRISPR-Cas13a Gemini System composed of two Cas13a:crRNA binary complexes, which can provide rapid ...
CRISPR-Cas13a系统用于肿瘤诊断与治疗的进展
CRISPR-Cas13a系统具有特异性识别并结合单链RNA序列从而非特异性切割RNA的特点，可应用于检测肿瘤外周血游离核酸，对早期肿瘤患者进行筛查。 同时，Cas13a在进行体内RNA切割的过程中，不涉及编码基因DNA的改变，可直接对基因转录产物mRNA进行编辑，达到基因修饰的目的，并能够同时靶向多基因转录产物从而调控基因的表达。 Cas13a系统可应用于分子诊断及RNA编辑中，该系统在肿瘤的诊断与治疗中也被证实具有广阔的发展前景。 基于已有的文献资料， …
Harnessing noncanonical trans-cleavage characteristics of Cas12 …
2024年10月12日 · Notably, while the CRISPR-Cas13a system integrates well with the RPA reaction 30 (Supplementary Fig. 12c, d), we observed that RT-RPA reagents inhibit the CRISPR-drCas12f1 system’s trans-DNase ...
Structure-guided discovery of ancestral CRISPR-Cas13 …
2024年7月18日 · The RNA-guided ribonuclease CRISPR-Cas13 enables adaptive immunity in bacteria and programmable RNA manipulation in heterologous systems. Cas13s share limited sequence similarity, hindering discovery of related or ancestral systems.
CRISPR-Cas13a system: A novel tool for molecular diagnostics
2022年12月8日 · Herein, we summarize the applications of the CRISPR/Cas13a system in the detection of pathogens, including viruses, bacteria, parasites, chlamydia, and fungus; biomarkers, such as microRNAs, lncRNAs, and circRNAs; and some non-nucleic acid targets, including proteins, ions, and methyl groups.
Insight into the Natural Regulatory Mechanisms and Clinical ...
6 天之前 · CRISPR-Cas, the adaptive immune system exclusive to prokaryotes, confers resistance against foreign mobile genetic elements. The CRISPR-Cas system is now being exploited by scientists in a diverse range of genome editing applications. CRISPR-Cas systems can be categorized into six different types based on their composition and mechanism, and there are also natural regulatory biomolecules in ...
Engineering CRISPR/Cas13 System against RNA Viruses: From …
2022年6月29日 · After three days post-transfection of the NS-crRNA-Cas13a complex and postinfection with dengue virus, the CRISPR-Cas13a system decreased viral RNA copy efficiently by 95%, providing a novel programmable anti-DENV strategy.