CD107a as a functional marker for the identification of ... - PubMed
Here we describe CD107a as a marker of NK cell functional activity using multi-parameter flow cytometry. CD107a is significantly upregulated on the surface of NK cells following stimulation with MHC devoid targets.
Relationship between CD107a expression and cytotoxic activity
Studies show that CD107a (LAMP-1) may be a marker for degranulation of NK and activated CD8+ T cells. In our study, the relationship between the expression of CD107a, cytokine secretion and cytotoxic activity in CD56+ NK, CD8+ T cells and …
CD107a Degranulation Assay to Evaluate Immune Cell …
In this chapter, we detail the steps required to isolate peripheral blood mononuclear cells (PBMCs), coculture them with target tumor cell lines, and evaluate the cytotoxic immune function by means of flow cytometry evaluation of CD107a expression on the surface of NK cells.
CD107a as a functional marker for the identification of natural killer ...
2004年11月1日 · Recently, lysosomal-associated membrane protein-1 (LAMP-1 or CD107a) has been described as a marker of CD8 + T-cell degranulation following stimulation. Here we describe CD107a as a marker of NK cell functional activity using multi-parameter flow cytometry.
Surface CD107a/LAMP-1 protects natural killer cells from degranulation ...
2013年8月22日 · In particular, CD107a/LAMP-1 has been widely used as a marker for degranulating cytotoxic lymphocytes, because surface expression is induced upon degranulation. 22-24 CD107a is a highly glycosylated protein comprising a 40-kDa backbone with predicted 17 N -glycosylation and 9 O -glycosylation sites.
CD107a Degranulation Assay to Evaluate Immune Cell Antitumor …
2018年11月22日 · Lysosome-associated membrane protein-1 (CD107a or LAMP-1) is a highly glycosylated transmembrane protein present in the lysosomes. In NK cells and cytotoxic T cells, CD107a is one of the most abundant proteins present in the lytic granules.
Ex vivo identification, isolation and analysis of tumor-cytolytic T ...
2003年10月5日 · We isolated pure, viable populations of tumor-cytolytic T cells directly from patient blood samples using flow cytometric quantification of the surface mobilization of CD107a—an integral...
The CD107 mobilization assay: viable isolation and ... - Nature
2005年3月3日 · CD107 mobilization assay enables detection of cytolytic NK cells. CD107a glycoproteins (as well as the related CD107b not indicated here) line the luminal surface of the outer membrane of lytic...
LAMP1/CD107a is required for efficient perforin delivery to lytic ...
Lysosome-associated membrane protein (LAMP) 1/CD107a is used as a marker for NK-cell degranulation, but its role in NK-cell biology is unknown. We show that LAMP1 silencing causes inhibition of NK-cell cytotoxicity, as LAMP1 RNA interference (RNAi) cells fail to deliver granzyme B to target cells.
NK Cells Activated through Antibody-Dependent Cell ... - Nature
2019年2月1日 · Activated NK cells expressed IFN-γ and CD107a. IFN-γ was detected at 24 hours (24 h) followed by a rapid decline; CD107a expression peaked at 48 h and persisted for >7 days.
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