Cas12a - Wikipedia
Cas12a (CRISPR-associated protein 12a, previously known as Cpf1) is a subtype of Cas12 proteins and an RNA-guided endonuclease that forms part of the CRISPR system in some bacteria and archaea. In CRISPR systems, Cas12a serves to destroy the genetic material of viruses and other foreign DNA, thereby protecting the cell from infection.
CRISPR/Cas系统的新星1——Cas12a - 知乎 - 知乎专栏
该蛋白家族中研究较多是Cas12a核酸酶，目前最常用的Cas12a蛋白来自氨基酸球菌属的BV3L6菌株和毛螺科细菌(LbCas12a)。 Cas12a蛋白已被广泛应用于包括细菌、酵母、植物和人类细胞在内的多个物种。
EnGen Lba Cas12a（Cpf1）核酸酶 - NEB
EnGen Lba Cas12a（Cpf1）核酸酶是一种可编辑 DNA 核酸内切酶，由单向导 RNA（gRNA）介导。. 靶向识别需要一段与目标位点互补的 gRNA，以及位于目标序列对侧的 DNA 链上的 5´ TTTV PAM（Protospacer Adjacent Motif）序列。. EnGen Lba Cas12a（Cpf1）核酸酶的切割位点位于 …
最全CRISPR系统介绍（张锋等大牛执笔，14张图看懂CRISPR全部内 …
除了靶DNA切割之外，类型V-A效应器Cas12a负责crRNA加工。 类型VI-A效应器Cas13a（C2c2）是RNA引导的RNA酶。 一旦RNA靶标识别被激活，Cas13a就成为非特异性的RNase，并且明显引起细胞毒性或死亡。
CRISPR-Cas12a: Functional overview and applications
Class II type V CRISPR-Cas12a is a new RNA guided endonuclease that has been recently harnessed as an alternative genome editing tool, which is emerging as a powerful molecular scissor to consider in the genome editing application landscape.
科普 | 浅谈CRISPR/Cas12基因编辑技术
2023年9月25日 · Cas12a. Cas12a(又称Cpf1)是一类由单个crRNA介导的核酸内切酶，能特异性识别并剪切带PAM(5-TTTN-3'或5'-TTN-3)的双链DNA(dsDNA)靶标，使DNA双链断裂并生成 黏性末端 。对单链DNA(ssDNA)靶标的识别和剪切不依赖PAM序列。
CRISPR/Cas12a基因组编辑技术及应用 - 知乎 - 知乎专栏
2024年1月3日 · CRISPR/Cas12a的特点. Cas12a属于CRISPR/Cas系统，具有被开发为基因组编辑技术的巨大潜力。CRISPR/Cas12a系统产生切割必须满足三个条件:Cas蛋白、crRNA和PAM。Cas蛋白是发挥识别、切割功能的蛋白，crRNA为靶向指引，PAM是CRISPR/Cas系统区分自我和入侵者的标记。
CRISPR-Cas12a: Functional overview and applications
2020年2月1日 · In this review we have sought to offer a condensed overview of the functionality of CRISPR-Cas12a, discussing the structural and functional features of the different stages of the reaction pathway leading up to the catalysis of the target DNA, and eventually discussing the applications of Cas12a in brief.
High-performance CRISPR-Cas12a genome editing for ... - Nature
2020年7月13日 · Cas12a (formerly Cpf1), an RNA-programmable DNA endonuclease similar to Cas9, has the potential to overcome these limitations. Unlike Cas9, Cas12a has intrinsic RNase activity that allows ...
Engineered CRISPR-Cas12a for higher-order combinatorial
2024年5月17日 · An engineered Cas12a enables higher-order combinatorial functional genomic screens using CRISPR interference. Multiplexed genetic perturbations are critical for testing functional interactions ...