Intracellular replication of the B. suis control strain with plasmid ...
Plasmid pMR10-CM was derived from pMR10 (obtained from R. Wright, Stanford University, Palo Alto, Calif.) and contained a chloramphenicol resistance gene inserted into the Sma I site of the...
A polysaccharide deacetylase enhances bacterial adhesion in …
2021年9月24日 · The pMR10-based constructs were transformed into α-select E. coli for screening and sequence confirmation before introduction into the host C. crescentus or H. baltica strains by electroporation.
Polar Localization of Replicon Origins in the Multipartite Genomes …
2003年6月1日 · Thus, we examined the localization pattern of a different replicon, the plasmid pMR10, which is an RK2-derived broad-host-range multicopy plasmid (R. Roberts and C. Mohr, unpublished results), as described in Materials and Methods.
Aconitate decarboxylase 1 participates in the control of pulmonary ...
2021年9月15日 · The aceA gene was then subcloned into the pMR10 plasmid (a kind gift from C.D. Mohr and R.C. Roberts, Stanford University) with the restriction enzymes BamHI and PstI. The resulting plasmid was introduced into E. coli strain …
Structure of pMR10. An ABA-inducible promoter complex
Secondly, the fused expression plasmid was constructed by inserting the cDNA fragment encoding the mature peptide of SAMDC into prokaryotic expression vector pET29a(+), and the positive ...
Overexpression of S-adenosylmethionine decarboxylase gene in rice ...
2002年11月1日 · The structure of plasmid pMR10 used for transformation is shown in Fig. 1. The cDNA of the SAMDC gene is located downstream of an ABA-inducible promoter complex [13]. The coding region of the bar gene is located downstream of the CaMV 35S promoter. The results of the transformation are summarized in Table 1.
Acidic pH triggers the phosphorylation of the response regulator …
2018年4月10日 · The plasmid encoding the mutant protein NtrX myc (D53A) was obtained from pMR10-NtrX myc by PCR amplification using primers 5′-GCTTTGCTGGTGCTGGCCATCTGGATGCAGG-3′ and 5′-CCTGCATCCAGATGGCCAGCACCAGCAAAGC-3 ′, followed by digestion with the enzyme DpnI.
2004年4月13日 · A Pxyl::mreB construct was generated by inserting the 2-kb region upstream of the xylose locus in front of the full-length mreB gene in the pMR10 low-copy plasmid (22), with an NdeI site engineered at the ATG (LS3808). This Pxyl::mreB plasmid was introduced into the mreB strains harboring the mreB cosmid.
NnrA Is Required for Full Virulence and Regulates Several
E. coli strains contain a pMR10 plasmid bearing nnrA (or a pMR10 without nnrA) and a pBBR-MCS1lacZ derivative, in which a promoter is fused to the lacZ reporter. It is expected that nnrA expression from pMR10-nnrA will interfere with a promoter-lacZ fusion if NnrA is able
Intracellular replication of the B. suiscontrol strain with plasmid...
Intracellular replication of the B. suiscontrol strain with plasmid pMR10-CM ( ) and of the strain with plasmid pBBR1-HLYK ( ) in differentiated human macrophage-like THP-1 cells. Experiments...