Live-cell imaging - Wikipedia
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century.
Imaging cell biology - Nature Cell Biology
Jul 27, 2022 · Imaging technologies drive discovery in cell biology. Innovations in microscopy hardware, imaging methods and computational analysis of large-scale, complex datasets can increase imaging...
Live-cell imaging powered by computation - Nature
Feb 20, 2024 · We specifically discuss how computational approaches can improve the signal-to-noise ratio, spatial resolution, temporal resolution and multi-colour capacity of live-cell imaging.
A quick guide to light microscopy in cell biology - PMC
Jan 1, 2016 · Light microscopy has several features that make it ideally suited for imaging biology in living cells: the resolution is well-matched to the sizes of subcellular structures, a diverse range of available fluorescent probes makes it possible to mark proteins, organelles, and other structures for imaging, and the relatively nonperturbing nature of ...
Cellular imaging | Nature Cell Biology
Sep 11, 2024 · A study now combines single-cell and spatial transcriptomics with imaging to infer the cellular composition and spatial architecture of specific niches.
Live Cell Imaging - CSH Protocols
New analytical options include live high-throughput/high-content screening in mammalian cells and computational analysis of live cell data. The manual presents hands-on techniques as well as background material, and can serve as a text in advanced courses.
Introduction to Live-Cell Imaging - Leica Microsystems
Apr 3, 2012 · Live-cell imaging provides spatial and temporal information of dynamic molecular events in single cells, cellular networks (in situ) or even whole organisms (in vivo). These features make live-cell imaging a requisite technique for addressing physiological questions in cell biology, cancer research, developmental biology and neuroscience.
How To Perform Fast & Stable Multicolor Live-Cell Imaging
Jul 26, 2024 · With the help of live-cell imaging researchers gain insights into dynamic processes of living cells up to whole organisms. This includes intracellular as well as intercellular activities. Protein or lipid trafficking, migrating immune cells, cellular organization in organoids, or developing animals are just a few examples. Live-cell imaging requires that the sample and …
Deep learning for cellular image analysis | Nature Methods
May 27, 2019 · Here we review the intersection between deep learning and cellular image analysis and provide an overview of both the mathematical mechanics and the programming frameworks of deep learning that...
Ultra-High Resolution 3D Imaging of Whole Cells - Cell Press
Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells.
Exploring Cell Imaging Techniques – Types, Analysis, and …
Cell imaging is a noninvasive way of tracking health-related attributes and disease-related abnormalities in cancer and other disorders. Cell imaging presents important information about the etiology, progression, and ultimate fate of diseases.
Using AI, images are fueling a new boom in cell biology
2 days ago · From left, Megan Molteni of STAT, Sarah Teichmann of the Cambridge Stem Cell Institute, Kun Zhang of Altos Labs and Anne Carpenter of the Broad Institute discussed the AI imaging revolution.
Live Cell Imaging - Molecular Devices
Live cell imaging is the study of cellular structure and function in living cells via microscopy. It is a fundamental research tool in cell biology labs and in a wide variety of industries. It enables the visualization and quantitation of dynamic cellular processes in real time.
The Cell Image Library
Accessible resource database of images, videos, and animations of cells, capturing a wide diversity of organisms, cell types, and cellular processes.
Super-resolution microscopy demystified - Nature Cell Biology
Jan 2, 2019 · Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
Live-Cell Imaging Techniques | Science Lab - Leica Microsystems
Jan 10, 2022 · Live-cell imaging provides, in real time, a “snapshot” of a cell’s or organism’s current state in its native environment without introducing artifacts typical of fixation. These capabilities make live-cell imaging a requisite technique for addressing questions in cell biology, cancer research, developmental biology, and neuroscience.
Long-term mesoscale imaging of 3D intercellular dynamics ... - Cell …
Sep 13, 2024 · Here, we introduce a compact real-time, ultra-large-scale, high-resolution 3D mesoscope (RUSH3D), achieving uniform resolutions of 2.6 × 2.6 × 6 μm 3 across a volume of 8,000 × 6,000 × 400 μm 3 at 20 Hz with low phototoxicity.
Center for Quantitative Cell Imaging – A Collaborative Biological ...
CQCI research emphasizes development and application of imaging approaches for molecules, cells and tissues. CQCI methods and approaches are shared with the UW campus and the scientific community beyond.
LIVECell—A large-scale dataset for label-free live cell ... - Nature
Aug 30, 2021 · Here, we present LIVECell, a large, high-quality, manually annotated and expert-validated dataset of phase-contrast images, consisting of over 1.6 million cells from a diverse set of cell...
ViewRNA ISH Assays | Thermo Fisher Scientific - JP
ViewRNA ISH Cell and Tissue Assays utilize in situ hybridization to visualize RNA with single-molecule sensitivity within individual cells and tissue sections. ... EVOS imaging systems incorporate high-resolution cameras, bright and digitally controlled LED light sources, and intuitive software to help you capture stunning, publication-quality ...
Cellular imaging - Latest research and news | Nature
Oct 13, 2024 · Cellular imaging encompasses the techniques that allow the detection and analysis of cellular organelles and macromolecules. Cellular imaging observations are obtained using...
Super-resolution 3D live cell imaging | Nature Methods
Mar 5, 2021 · 3D pRESOLFT enables volumetric live cell imaging with high spatial and temporal resolution. Super-resolution imaging of fixed cells in 3D is well established.
Single-cell imaging - Latest research and news | Nature
Oct 7, 2024 · Single-cell imaging is an ensemble of microscopy techniques that enable the collection of separate data from individual cells, as opposed to values averaged over populations of cells.