DNA ligase - Wikipedia
DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary ...
DNA Ligase- Definition, Structure, Types, Functions
2023年8月3日 · DNA ligase plays a vital role in DNA replication, DNA repair, and DNA recombination. Now day purified DNA ligase is isolated in the laboratory, which is used in gene cloning to join DNA molecules together to form recombinant DNA.
DNA Ligase - an overview | ScienceDirect Topics
DNA ligase is an enzyme that plays a crucial role in maintaining the structural integrity of the genome by repairing breaks in the DNA backbone. It catalyzes the formation of a bond between adjacent nucleotides in the DNA, ensuring the stability of genetic information.
DNA Ligation Overview: Mechanism, Types, Applications ...
DNA ligation is the process of joining two strands or fragments of DNA molecules through a phosphodiester bond formed by the enzyme DNA ligase. DNA ligase is involved in maintaining the structural integrity of genomes by participating in DNA replication or repair processes.
DNA Ligase: Structure, Mechanism, and Function | Science - AAAS
DNA ligase is therefore an essential enzyme required for normal DNA replication and repair in E. coli. Purified DNA ligases have proved to be useful reagents in the construction in vitro of recombinant DNA molecules.
Eukaryotic DNA Ligases: Structural and Functional Insights
DNA ligases are required for DNA replication, repair, and recombination. In eukaryotes, there are three families of ATP-dependent DNA ligases. Members of the DNA ligase I and IV families are found in all eukaryotes, whereas DNA ligase III family members are restricted to vertebrates.
DNA Ligases: Progress and Prospects - PMC
2009年6月6日 · DNA ligases seal 5′-PO 4 and 3′-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are essential guardians of genomic integrity, and ligase …